Support for research on fertility

We are launching support interesting and innovative student scientific projects that deserve reliable collateral, but so far they had stable funding. You can now help with the support of science and research. We are logged on as the first research into the impact of latent toxoplasmosis on human fertility. The aim is to select for the year 2017 for this project a total of 100 thousand. You can follow the procedure here. You can contribute any amount using a credit card (see the button in the right sidebar) or bank order on behalf of Andriasu 2001182781/2010, variable symbol, 1701. In the notification, you can give the e-mail or phone for communication regarding the sending of the confirmation of the donation for tax deduction (or you can always contact us contact email). Below is a brief extract from the project for the grant agency of the Charles University, prepared as an annex to the application for a grant to the Association administered Andrias. Opinions on the project can be read here.

Jana Hlaváčová, author of the project.

The Czech name of the project: the effect of latent toxoplasmosis on fertility in humans
The English name of the project: Influence of latent toxoplasmosis on fertility in humans
The current investigator: Bc. Jana Hlaváčová

Toxoplasma gondii in developed countries is probably one of the most common parasitic protozoa in humans. Recent studies suggest that t. gondii affects the fertility of the host. Women with toxoplazmózou more often undergo artificial insemination. In men with lower concentrations has been observed toxoplazmózou and sperm motility. T. gondii was found in semen and reproductive organs of various animals. In several species of animals is confirmed by its transmission sexually. These findings lead to the conclusion that Toxoplasma gondii has a negative impact on the reproductive function of the host.
The aim of this project is to compare the fertility problems, and the success rate of artificial insemination with infected and nenakažených individuals. We also want to find out if toxoplasmosis could be a sexually transmitted disease in humans. Through cooperation with more work on the issue of how we look from a clinical and scientific point of view and connect the views of Parasitology, evolutionary psychology, and reproductive biology. The project will build on the study of the impact of latent toxoplasmosis on human reproductive function, which takes place on our faculty for several years.

The objectives of the project
The main objective of this project is to investigate the effect of latent toxoplasmosis on reproductive function in humans. Due to the fact that this issue is very complex, presented by several project objectives:
1. Compare fertility problems for infected and nenakažených individuals, to study the causes of the problems with getting pregnant and also the reasons that lead to artificial insemination.
2. Compare the prevalence of toxoplasmosis in women who have undergone artificial insemination with prevalence in women who became pregnant the natural way.
3. Compare the success rate of treatment and artificial insemination with infected and nenakažených women.
4. Compare the prevalence of toxoplasmosis in the pair. Determine whether there are more often infected with both individuals of the couple or is infected by just one of them.
5. Compare the prevalence of toxoplasmosis in men with fertility problems with prevalence in men with no problems.
6. Find out what T. Toxoplasma gondii effect on the concentration of motile sperm count in men and stationary.
7. Compare the effect of Toxoplasma on fertility depending on RhD genotype.
8. Cooperation with different workplace to link knowledge of multiple scientific disciplines, and to look at the issue of how to study from a clinical and scientific point of view.

The method of solution
The project will be addressed in the form of prospective studies. We work together with Gynecological-obstetric emergency clinic 1. the Faculty of Medicine of Charles University and the General faculty hospital in Prague and other workplace. All the data will be processed in anonymised form. Below are described in more detail each part of the project.

1. Prospective study
a. Case-control study I.
In cooperation with Obstetrics-obstetrics clinic 1. the Faculty of Medicine of Charles University and the General faculty hospital in Prague we will compare the prevalence of toxoplasmosis in women who visited a Centre for assisted reproduction (CAR) with the problem of getting pregnant, with prevalence in women who became pregnant the natural way. Checks will be collected at the same time collecting samples in the CAR, according to age, place of residence and the number of previous pregnancies, and their choice will help Ing. Drahomira Springer, Ph.d. from the Institute of Medical Biochemistry and laboratory Diagnostics (ÚLBLD) from the General faculty hospital in Prague. According to the supplied parameters should look for the control group and passes us anonymous serum. The control file will be selected from the file of the women who undergo screening for thyroid disease in 1. trimester of pregnancy and sign informed consent to use their frozen sera for other scientific purposes.
(b). In cooperation with the above mentioned clinic we will create all the mothers group, which visited the CAR with the problem of getting pregnant and we'll compare the success in getting pregnant for Toxoplasma-positive and Toxoplasma-negative women. We will have to evaluate the number of IVF attempts, the number of healthy eggs, the number of collected, the number of successfully fertilized eggs and the number of transferred embryos.
(c). We have a spermiogramy men who have stayed at the CAR. We will study the effect on the concentration of Toxoplasma gondii t. moving and stationary of the sperm. We'll compare the concentration of motile sperm in stationary and infected and nenakažených men with fertility problems, and also in men with normal sperm count.
(d). Case-control study (II).
We'll compare the prevalence of toxoplasmosis in men with fertility problems with prevalence in men with no problems. Divide into groups of men with normal sperm count and the pathological and the prevalence in these two groups are compared.
and. We will study the possible role of sexual transmission of t. gondii. We compare the prevalence of toxoplasmosis in the pair. CAR visits per week approximately 10 pairs with a problem getting pregnant. We will see if they are significantly more likely to be infected by both partners in a pair of toxoplazmózou than would correspond to the frequency of the disease in men and women. Couples are on their first visit will be to fill out the questionnaire focused on epidemiological factors that contains questions concerning, for example, the size of the place of residence, contact with garden soil, ingestion of poorly washed vegetables or exposure to risk of sexually transmitted diseases.
(f). We study the influence of Toxoplasma on fertility of people depending on their RhD genotype. Divide individuals into groups depending on whether you are Rh positive, Rh positive homozygoti heterozygotes or Rh negative homozygoti and also depending on whether or not they are infected with toxoplazmózou, and in these groups are compared the medical histories of patients, records about IVF and the results from spermiogramů.

Volunteers will be enrolled in a prospective study, on the basis of the signed informed consent, in which you will be familiar with the general objective of the study and adopt the possibility of testing their biological material.

Prospective study will be very time consuming. Collection and processing of blood samples will be carried out regularly 2-3 times a week and will participate in the main investigator on it along with the Bc. Catherine Fiuraškovou.
All Sera from subjects we will test for the presence of antibodies to t. gondii in the EAA, in cooperation with the national reference laboratory for tropical regions complement fixation method (KFR) and at the same time, we will establish the ELISA IgG subclass antibodies and in the case of high levels of IgG and IgM and IgA antibodies. The results of prevention we consulted with RNDr. Petr Kodymem, CSc. of the national reference laboratories in the EAA. Suspected acute prevention during the study is backed by cooperation with MUDr. Margaret Geleneky from the clinic of infectious, parasitic and tropical diseases in the hospital Na Bulovce, which we will forward to potential patients to further consultations and possible treatment. Due to the fact that women are included in the study are planning to become pregnant, the test results on prevention will be the main investigator on the fly to pass up to the head of the doctors CAR 2 weeks of blood sampling.

Blood samples for genotyping of RhD will be tested using PCR master researcher on a specialized workplace led by RNDr. Emanuel Bivouac, CSc. To the processing of data from a medical database and from an epidemiological questionnaire will be the main řešitelce to help Bc. Catherine S.

The association between the incidence of toxoplasmosis in partner and partner we will analyze the use PivotTable reports, while the strength of the effect we will be expressed as odds ratio. Other results we statistically analyzed using general linear models, to which we as kovariáty variables that could affect the study issues (e.g.. age). In the event that the data will not have a normal distribution, we can use to test the non-parametric tests (e.g.. partial Kendall, which allows you to include in the test model 3. variable, here the covariate age). The success of becoming pregnant will be analysed on the basis of clinical data. It could be affected by the different intensity of the efforts of the pairs of good result, however, we assume that the voluntary visiting vocational workplace with this intensity for couples will not differ significantly.

2. Interview study
Collection of data from a prospective study is time-consuming. We had the opportunity to get some published results, or the ability to draw up the first publication already in the first year of the project, we create a parallel with the launch of the above prospective study and a detailed questionnaire and start the anamnestický a cross-cutting study. This will be focused, first, on the study of the relationship of toxoplasmosis and fertility, and, secondly, to study the possibility of sexual transmission of the disease. The questionnaire will include for example. questions followers health or other issues women from pregnancy, as long as the women tried to have a baby, if you have undergone artificial insemination and also questions that could be related to sexual transmission, for example. the number of unprotected sexual relations. The questionnaire will be distributed via the Web and Facebook page "Pokusní rabbits". This group was created by members of our laboratory, and currently includes more than 13500 volunteers, of which about 2500 have in the past been examined for tropical regions. The research will be used to study the environment, and a custom Web interface Qualtrics to collect anonymous data. We assume that the main benefit of the operational methods will be charting the study issues in a short time on a larger data set, and that this method will also discover any dependencies that are so far overlooked. On the basis of past experience, we expect that the results of the questionnaire study will help improve and at the same time running a prospective study in the Centre of assisted reproduction.

The timing during the study
When the expected number of 500 pairs involved in the prospective study will be collecting the samples run at least the entire first year of the project, while the collection rate will depend on the willingness and cooperation of the nurses in the Emperor and the couples themselves. We expect, therefore, that the collection may extend well into the following year. During the processing and testing of the samples and the handling of records from a medical database, we'll create the above-mentioned questionnaire and we will publish interesting results already in the first year. In the coming years we will be outside the aforementioned publish and present the results of a prospective study.

Characteristics of the team: Bc. Jana Hlaváčová is a student of the 2. year of the graduate field of Theoretical and evolutionary biology in the Department of philosophy and history of science. The Coordinator and the main Solver project. In 2015, she defended her thesis focused on the influence of t. gondii on the reproductive function of the host. This issue is currently being spent on your master studies. In the year 2017 will be defending a thesis on the study issues and establishes in the doctoral study.
RNDr. Šárka Kaňková, Ph.d. works at the Department of philosophy and history of science, Faculty of science, and in particular deals with the theoretical and evolutionary psychology. More than 10 years is devoted to the impact of latent toxoplasmosis on the course of pregnancy and fitness of offspring. She was the author of 12 influential publications (h = 9). Will keep an eye on the proposed project, will assist in statistical data processing and preparation of publications.
Prof. RNDr. Jaroslav Flegr CSc. works at the Department of philosophy and the history of the Natural Sciences Faculty, his specialization is theoretical and evolutionary biology, psychology, and parasitology. So far the principal investigator or guarantor of more than 25 successful grant projects and the author of the influential publication 129 (h = 33). Will assist in statistical data processing.
BC. Katerina Fiurášková is a student of 1. year of the master's Zoology. In their bachelor's thesis dealt with the influence of hormonal contraceptives and steroid hormones to partner preferences of women. Currently on this issue follows and covers the impact of hormonal contraceptives on partner selection and subsequent satisfaction in the relationship. In the proposed project will contribute mainly to the collection and processing of blood samples.
BC. Catherine S is student 3. year of the master's field of Theoretical and evolutionary biology in the Department of philosophy and history of science and deals with the relationship between morphological characteristics and personality. In the proposed project will participate in mainly on the processing of medical records database and epidemiological questionnaire.

Presentation of the results
From this research should produce at least 3 work published in influential magazines. The publication shall contain the number and dedication of the project GA CU affiliates. Members of the research team plan to present the results at a Conference of the Czech and Slovak Ethological society and also at the Conference "Problems of Toxoplasma" held in 1211 in Prague.
The results will also be part of the thesis the master Solver Bc. Jana Hlaváčová, work will be shown appreciation for support, including the number of the grant project GAUK. Interesting and the results will be published in cooperation with the Department for external relations of the Faculty of popularizovány in appropriate media.

Given the importance of the issue of fertility, which currently refers to approximately 20% of couples, continuity is assumed to be additional research that will build on the knowledge gained through this project.



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